Now, pipette 1 ml (or weigh 1 gram) of the dilution in #2 (10-4 or 100 ppm) into a clean 1-liter volumetric flask and fill to the mark with system water. You now have a 10-4, or 100-ppm, dilution. Next, pipette 1 ml (or weigh 1 gram) of the dilution in #1 (10-2 or 10 ppt) into a clean 100-ml volumetric flask and dilute to the mark with distilled water. Note: You could obtain the same -concentration by weighing 10 g into a 1-liter flask, or 20 g into a 2-liter flask. (Be sure to mix thoroughly the powders can be difficult to mix.) You now have a 10-g/liter (10-ppt, 10-2) -concentration of your tracer. Then dilute to the mark with distilled water. Using an accurate laboratory scale, weigh 1 gram of dye directly into a 100-ml volumetric flask. first, prepare a 100-fold dilution by weight.
To prepare a 100-ppb standard of rhodamine B or other dye in powder form: 1. This study involving rhodamine b is a milestone for me, because I believe through the data I present here, that I have reached two of my main goals, one was to test the accuracy and quality of the spectrometers I built and worked with and two, accuracy and quality sample preparation on the chemistry side of things. I certainly made it a goal to learn and perfect my techniques so to improve the quality and reliability of the data I collected and presented. I have a chemistry and engineering background, but this was a very challenging endeavor. I started doing this a little over 5 months ago, with no experience in spectrometers or their usage, only a vague understanding in their operation.
Quality sample preparation is a critical element in the analysis of spectral data, so too, is the knowledge and techniques administered by the individual doing the analysis.